International Journal of Academic Research and Development

International Journal of Academic Research and Development


International Journal of Academic Research and Development
International Journal of Academic Research and Development
Vol. 6, Issue 3 (2021)

Preparation and standardization of locally produced antibiotic multidiscs in Benin City, Nigeria


George Akintinde Olajide, Helen Ogefere

Locally manufactured antibiotic sensitivity discs are becoming increasingly used in both private and public Health Institution Laboratories in Nigeria. This study aimed to prepare and standardize locally produced antibiotic multidiscs in Benin City, Nigeria. Dye cut blade with eight (8) finger holes of approximately six (6mm) diameter was constructed manually and the printed alabaster paper discs were placed on the Herzberg 13 x 18 die-cutting impression machine to bring out the multidiscs shape. Antibiotic discs named Boljid Tripple T multidiscs (Boljid) were prepared by impregnation of antibiotic solution onto 300 gram alabaster paper. The different antibiotic tablets (Ciprofloxacin-5ug, Ofloxacin-5ug, Azithromycin-15ug, Augmentin-30ug, Pefloxacin-10ug and Levofloxacin-5ug) and injections Ceftriaxone-30ug, Gentamicin-10ug/ml, Cefuroxime-30umg, Nitrofurantoin-300ug and Ceftazidime-30ug were used for Boljid multidisc preparation. Control organisms and clinical isolates was obtained from the Lagos State University Teaching Hospital, Idi Araba, Lagos and University of Benin Teaching Hospital, Benin City, Edo State respectively. The prepared Boljid multidiscs, were standardized by comparing the efficacy with the commercially available multidiscs namely Abtek, Celtech, Divine Favour, Maxi High profile and Oxoid single discs. Triplicate plates were prepared for each of the multidiscs and tested against the test organisms using the Kirby-Bauer disc diffusion method. The test organisms investigated included clinical isolates of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella species, Proteus species and control strains of Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853. Results obtained showed that for Ciprofloxacin, Boljid (25.00± 0.58mm) and Celtech (25.50± 0.58mm) multidiscs produced similar inhibition zone diameter against ATCC Staphylococcus aureus. Boljid (21.67±0.33mm), Divine Favour (20.00±1.15mm) and Oxoid disc (20.00±0.00mm) produced similar zone of inhibition against Pseudomonas aeruginosa. For Gentamicin, Boljid, Abtek, Celtech and Maxi multidiscs produced no zone of inhibition against ATCC Pseudomonas aeruginosa while Divine Favour (11.67±0.58mm) and Oxoid (20.00±0.58mm) produced inhibition zones that were significantly different. Boljid, Abtek, Celtech, Divine Favour and Maxi multidiscs produced no zone of inhibition against the clinical isolates of Escherichia coli. Boljid (14.00±0.00mm) and Oxoid discs (14.67±0.00mm) produced similar zone of inhibition against clinical isolate of Klebsiella species. For Ofloxaxin, Boljid and Oxoid discs produced no zone of inhibition against the clinical isolate of Pseudomonas aeruginosa. Boljid multidiscs produced locally, compared favourably with Oxoid, Abtek, and Celtech used in this study and therefore recommended for product certification for routine Laboratory use.
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How to cite this article:
George Akintinde Olajide, Helen Ogefere. Preparation and standardization of locally produced antibiotic multidiscs in Benin City, Nigeria. International Journal of Academic Research and Development, Volume 6, Issue 3, 2021, Pages 33-35
International Journal of Academic Research and Development International Journal of Academic Research and Development