International Journal of Academic Research and Development

International Journal of Academic Research and Development


International Journal of Academic Research and Development
International Journal of Academic Research and Development
Vol. 4, Issue 1 (2019)

Evaluation of Microbiological Profile and Antibiotic Susceptibility Pattern of Aerobic bacterial isolates in women of reproductive age group with vaginal infections attending tertiary care teaching hospital in Solapur, Maharashtra


Dr. Anu Sharma, Dr. Shaikh Najmeen Abdul Rauf, Dr. DP Danave, Dr. KV Ingole

Background: Vaginal microbiota is diverse and complex comprising of dozens of microbiological species in variable quantities and proportions. Alteration in microbial flora and infections caused by them may remain undiagnosed due to negligence, hesitation and shame among females, resulting in various Gynecological and Obstetrical complications. Aim of the study is to evaluate microbiological profile of organisms isolated from women’s suspected of vaginal infections and to determine the antibiotic susceptibility pattern of aerobic bacterial isolates. Material and Method: A total of 250 women of reproductive age group attending tertiary care hospital with signs and symptoms suggestive of vaginal infection were included in the study. Study was conducted from June 2018 to December 2018. Two high vaginal swabs were taken using all aseptic precautions, and transferred immediately to the laboratory. One swab was used for microscopic evaluation i.e. wet mount and gram stain for evaluation of bacteria, fungi, trophozoite of Trichomonas vaginalis, Clue cell. Second swab was used for culture for aerobic bacteria and fungi and identification was done using standard Microbiological techniques. Results: Out of 250 women, 239 had vaginal infection. In the present study most common age group affected was 25-30 years (50.20%) followed by 31-35 (31.4%) age group, and 15-20 years (18.41%). Most common cause of vaginal infection were aerobic bacteria of which gram negative bacteria n=133 (76.43%) were most commonly isolated. Among gram negative bacteria E.coli 80 (46%) were most common cause of vaginal infection followed by Klebsiella pneumoniae 30 (17.24%), Acinetobacter baumannii 20 (11.5%), Proteus vulgaris 3 (1.72%). Among gram positive bacteria (n=41) Staphylococcus aureus 31 (75.60%) were most commonly isolated, of which MRSA 15 (48.4%), MSSA 13 (41.93%), MRCONS 6 (19.35%), MSCONS 1 (3.22%) were seen. Other gram positive bacteria seen was Enterococcus faecalis 10 (6.75%).Significant number of women were infected with Candida spp. 34 (13.6%) followed by Trichomonas vaginalis 20 (8.0%) and Gardnerella vaginalis 11(4.4%). Antibiotic susceptibility of gram negative bacteria (n=133) showed overall high sensitivity to Meropenem 82% followed by Amikacin 75%, Piperacillin-tazobactam 58.4%, Ciprofloxacin 45.4% and Cefotaxime 30%. In case of gram positive bacteria n=41 (100%) sensitivity was seen for Vancomycin and Linezolid, Gentamicin 75%, Clindamycin 70%, and Ciprofloxacin 54%.
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