Identification of female specfic protein by immunoelectrophoresis in the house cricket Gryllodes sigillatus walker (Gryllidae)
Dr. H Faritha Begam
Using immunological tools an analysis was made on the identification of female specific protein during the first reproductive cycle of House cricket Gryllodes sigillatus. The reproductive cycle of the insects were divided into three phases based on the accumulation of vitellogenin, namely vitellogenic, previtellogenic and postvitellogenic phases. The oocyte antigens were purified and inoculated into rabbits to raise the whole oocyte antigens. The antiserum was collected and absorbed with male haemolymph proteins to obtain the antiserum for the female specific proteins. Using immunoelectrophoretic studies, both whole and specific-antisera were made to react with protein extracts of fatbody, haemolymph and ovary during the different phases of reproductive cycle . Immunoelectrophoresis of control vitellogenic fat body and ovary extracts against anti-female haemolymph serum revealed 7 and 8 protein precipitin arcs respectively. While the same antigens agsinst anti-oocyte serum revealed 6 and 7 fractions respectively. Immunoelectropherogram of anti-female haemolymph serum adsorbed with male haemolymph against control vitellogenic fat body and ovary each produced a single procipitin line, thus confirming that the synthesis of FSP was extraovarian. The reaction of anti-oocyte serum adsorbed with male haemolymph against control vitellogenic fat body and oocyte extract showed one and two precipitin protein arcs respectively and thus confirmed that the synthesis of ESP was of intraovarian in origin.