International Journal of Academic Research and Development

International Journal of Academic Research and Development


International Journal of Academic Research and Development
International Journal of Academic Research and Development
Vol. 2, Issue 2 (2017)

A comparative study on alkaline protease production from Bacillus spp. and their biodegradative, dehairing and destaining activity


Basheer Thazeem, Goldy Primo Beryl, Mridul Umesh

Proteolytic Bacillus strains isolated from tannery effluent were screened on saline skim milk agar plates (pH-9) for their potency to secrete alkaline protease. Bacillus licheniformis exhibited highest extracellular alkaline protease activity of 360 Units/ml at 72hrs of incubation. Among the proteolytic isolates, Bacillus licheniformis degraded chicken feathers to the maximum extent (58%), followed by Bacillus cereus M-1 (40%) and Bacillus subtilis (37%). Dehairing activity of alkaline protease revealed that they were efficient when employed in dip method, than spray and paint method. Complete destaining was observed within 20mins by the crude enzyme produced from Bacillus licheniformis. Crude enzymes were partially purified by acetone precipitation and SDS-PAGE results revealed that the molecular weight of the alkaline protease enzyme was 28kDa.
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How to cite this article:
Basheer Thazeem, Goldy Primo Beryl, Mridul Umesh. A comparative study on alkaline protease production from Bacillus spp. and their biodegradative, dehairing and destaining activity. International Journal of Academic Research and Development, Volume 2, Issue 2, 2017, Pages 74-79
International Journal of Academic Research and Development International Journal of Academic Research and Development