Vol. 5, Issue 1 (2020)
Determination of Polyalcohols from cassia auriculata Linn: Periodate oxidised seeds polysaccharide by smith degradation method
Author(s): RB Singh
Abstract: Water soluble seeds polysaccharide was extracted from Cassia auriculata Linn. on acid hydrolysis with sulphuric acid and obtained hydrolysate on paper chromatographic analysis led to separation of D-galactose and D-mannose in 1:3 molar ratio. Purified seeds polysaccharide was reduced after periodate oxidation with sodium borohydride and sulphuric acid by Smith degradation method. The obtained hydrolysate produced polyalcohols as glycerol and erythritol in 1:2.96 molar ratio by paper chromatographic analysis. The derivatives of polyalcohols were produced from seeds polysaccharide as glycerol-tri-O-p-nitrobenzoate and tetra-O-tosyl-erythritol. The absorbance of polyalcohols were recorded in photoelectron colorimeter at 540 mµ for glycerol and erythritol.