Periodate oxidation method used for the confirmation of water soluble seeds polysaccharide structure from Cassia glauca Lam. plant
Cassia glauca Lam. seeds yielded a water soluble polysaccharide on acid hydrolysis as D-galactose and D-mannose in 1:4 molar ratio. Upon periodate oxidation of seed polysaccharide with sodium metaperiodate, it consumed 1.15 moles of periodate with simultaneous liberation of 0.23 moles of formic acid per mole of anhydrohexose unit after 60 hrs. On the basis of methylation results, the proposed seeds polysaccharide structure has been confirmed by periodate oxidation method.