Vol. 3, Issue 3 (2018)
Antimicrobial activity and phytochemical analysis of aerial parts of Cynodon dactylon
Author(s): Dhanapal Venkatachalam, Samuel Thavamani B, Muddukrishniah K, Suganya Vijayan, Vinod KR
Abstract: Objective: The present study was designed to check in-vitro efficacy of Cynodon dactylon plant extracts against selected bacterial and fungal strains The Cynodon dactylon (Family: Poaceae) commonly known as “arugum pillu” (Tamil), popularly known as Bermuda grass grows throughout India. Methodology: Ethanol and aqueous extracts of Cynodon dactylon was used for antimicrobial screening. Antibacterial activity was tested against four pathogenic bacterial strains i.e. Streptococcus aureus (Gram+) (MTCC737), Pseudomonas aeuriginosa (Gram-) (ATCC27853), Salmonella paratyphi, Enterobacter aergens while antifungal activity was tested against two fungal strains i.e. Candida albicans (ATCC2091), Aspergillus niger. (ATCC 6275). Anti-bacterial activity of Cynodon dactylon extract was carried out by conventional disc plate method. Results: Ethanolic extract of C.dactylon showed the largest zone of inhibition (1.1cm) against Salmomnella paratyphi at 50µgm/ml and 0.9 cm zone of inhibition against S.aures, P.aeruginosa and E.aerogens at 50µgm/ml. Aqueous extract of C.dactylon showed 0.8cm zone of inhibitions against S.paratyphi and E.aergens at 15µgm/ml. Aqueous extract showed 0.7 cm and 0.6cm zone of inhibition against S.aureus and P.aeruginosa respectively at 15 µgm/ml. Ethanolic extract has shown more potent antibacterial activity than aqueous extract. Ethanolic extract was found to be more effective against fungus Candida albicuns than aqueous extract. The zone of inhibition of ethanolic extract was 2.6 cm and the zone of inhibition of aqueous extract was 1.7cm.Aqueous extract was found to be more effective against fungus Aspergillus niger than ethanolic extract. The zone of inhibition of aqueous extract was 3,2cm and the zone of inhibition of ethanolic extract was 2,0 cm.The phytochemical screening demonstrated the presence of different types of compounds like terpenoids, tannins, and flavonoids which may contribute for the anti-microbial action of this plant, Conclusions: These findings provide scientific evidence of traditional use Cynodon dactylon and also indicate the potential of this plant for the development of antimicrobial agents.