Klebsiella spp. are common Gram-negative pathogens, frequently encountered in nosocomial infections. The present study was carried out to evaluate the prevalence and development of resistance in Klebsiella spp against commonly used antibiotics.
Materials and methods
Klebsiella spp were isolated from various clinical samples such as Miscellaneous (733 which includes sputum, High vaginal swab, Drainage Tube, Bronchial Wash, Stool, eye swab, throat swab, pus) and urine (338) were collected from January 2015 to December 2015 in SSSMCRI. This bacterial species were characterized and identified according to standard bacteriological methods, Gram Staining and biochemical tests. Antimicrobial susceptibility test was performed by Kirby – Bauer disk diffusion method to determine the antibiotic resistance pattern.
Klebsiella spp. were isolated from 733 miscellaneous samples in which 60% were Klebsiella pneumoniae and 40% were Klebsiella oxytoca whereas from 338 urine samples Klebsiella pneumonia 56% were more prevalence than Klebsiella oxytoca 44%. Our findings specify that most of the Klebsiella infections were caused by K. pneumoniae followed by Klebsiella oxytoca. Hence K. pneumoniae is the most common etiologic agent of nosocomial infection.
In the present study, most of the isolates of Klebsiella spp were found to have intrinsic resistance to ampicillin, Cefazolin and Nitrofurantoin in both the samples. But also we proved and recommended Amikacin and Imipenem are the most effective antibiotics being 100% and 96% effective against K. pneumoniae strains.